분자세포생화학 연구실은 ** 진핵세포 mRNA 번역 개시(translation initiation) ** 의 기초적인 메커니즘과 이를 조절하는 핵심 결합 단백질들의 역할에 대해 심도 있게 연구합니다. 특히, 우리는 선천 면역, 자폐증, 학습과 기억과 같은 생리학적 및 병리학적 과정에서 mRNA 번역 조절이 어떻게 이루어지는지에 집중하고 있습니다. 이를 통해 이러한 질병들이 발생하고 진행하는 분자적 기초를 이해하고, 더 나아가 새로운 치료 전략을 개발하는 데 기여하고자 합니다.

1. 4EHP-GIGYF1/2에 의해 조절되는 mRNA 레퍼토리 분석
2. 결합 동역학 분석을 통한 번역 개시 단계 실시간 모니터링
3. ASD (자폐스펙트럼장애) 관련 GIGYF1/2 변이가 단백질 번역 환경에 미치는 영향 연구

- SELECTED PUBLICATIONS

• Ladak RJ*,ChoiJH*, Luo J, Chen OJ, Mahmood N, He AJ, Naeli P, Snell PH, Bayani E, Hoang HD, Alain T, Teodoro JG, Wang J, Zhang X, Jafarnejad SM, Sonenberg N (2024) The 4EHP-mediated translational repression of cGAS impedes the host immune response against DNA viruses. (* co-first author) Proc. Natl. Acad. Sci. U. S. A., 121 (48) e2413018121
• Choi JH*, Marsal-Garcia L*, Peraldi E, Walters C, Huang Z, Gantois I, Sonenberg N (2024) Early metformin treatment: An effective approach for targeting fragile X syndrome pathophysiology.Proc. Natl. Acad. Sci. U. S. A.,121 (31) e2407546121
• Mahmood N*,ChoiJH*, Wu PY, Dooling SW, Watkins TA, Huang Z, Lipman J, Zhao H, Yang A, Silversmith J, Inglebert Y, Koumenis C, Sharma V, Lacaille J-C, Sossin WS, Khoutorsky A, Mckinney A, Costa-Mattioli M, Sonenberg N (2024) The ISR downstream target ATF4 represses long-term memory in a cell type-specific manner. (* co-first author) Proc. Natl. Acad. Sci. U. S. A.,121(31) e2407472121
• Choi JH*, Luo J*, Hesketh GG, Guo S, Pistofidis A, Ladak RJ, An Y, Naeli P, Alain T, Schmeing T.M, Gingras AC, Duchaine T, Zhang X, Sonenberg N, Jafarnejad SM (2024) Repression of mRNA translation initiation by GIGYF1 via disrupting the eIF3-eIF4G1 interaction.Science Advances, 10 (29), ead15638
• Zhang X*, Choi JH*, Dai D, Luo J, Ladak R, Li Q, Wang Y, Zhang C, Wiebe S, Liu A, Ran X, Yang J, Naeli P, Garzia A, Zhou L, Mahmood N, Deng Q, Elaish M, Lin R, Hobman T, Pelletier J, Alain T, Vidal S, Duchain T, Mazhab-Jafari M, Mao X, Jafarnejad SM, Sonenberg N (2022) SARS-CoV-2 impairs interferon production via NSP2-induced repression of mRNA translation. (* co-first author) Proc. Natl. Acad. Sci. U. S. A., 119(32): e2204539119
• Choi JH, Kim SH, Jeong YH, Min KT, Kim KT (2019) hnRNP Q regulates Cap-independent Fmrl translation in neurons. Molecular and Cellular Biology, 39(4), e00371-18 _Selected "Spotlight article"
• Choi JH, Wang W, Park DK, Kim SH, Kim KT,Min KT (2018) IRES-mediated translation of cofilin regulates axonal growth cone extension and turning. The EMBO Journal, 37(5), e95266

- CO-AUTHORED PUBLICATIONS

• Huang Z, Wiebe S, Nagpal A, Choi JH, Walters C, Mahmood N, Khoutorsky A, Lacaille JC, Sonenberg N (2024) Dysregulating mTORC1-4E-BP2 signaling in GABAergic interneurons impairs hippocampus-dependent learning and memory. Learning & Memory, 31 (10-11), a054018
• Wang P, Li Z, Kim SH, Xu H, Huang H, Yang C, Snape A, Choi JH, Bermudez S, Boivin MN, Ferry N, Karamchandani J, Nagar B, Sonenberg N (2024) PPM1G dephosphorylates eIF4E in control of mRNA translation and cell proliferation. Life Science Alliance, 7(10),e202402755
• Kim SH,ChoiJH, Marsal-García L, Amiri M, A Yanagiya, and Sonenberg N (2023). The mRNA translation initiation factor eIF4G1 controls mitochondrial oxidative phosphorylation, axonal morphogenesis, and memory. Proc. Natl. Acad. Sci. U. S. A., 120(25): e2300008120
• Zhang X, Chapat C, Wang P, Choi JH, LiQ, LuoJ, WiebeS, KimSH, RobichaudN, Karam IF, Dai D, Hackett AP, Lin R,Alain T, Yang L, Jafarnejad SM, Sonenberg N (2021).microRNA-induced translational control of antiviral immunity by the cap-binding protein 4EHP. Molecular Cell, 81(6), 1187-1199
• Kim SH, Choi JH, Wang P, Go CD, Hesketh GG, Gingras AC, Jafarnejad SM, Sonenberg N (2021) Mitochondrial Threonyl-tRNA synthetase TARS2 is Required for Threonine-sensitive mTORC1 Activation. Molecular Cell, 81(2), 398-407.e4